Fig. 3: Transcriptome responses of microalgae exposed to heat stress and nanoplastics.

a PCA analysis. b Venn analysis. c Venn analysis. d Heatmap of differentially expressed genes clustering. e Expression of genes associated with pyruvate metabolism. f Expression of genes associated with heat shock proteins. g Schematic representation of the adverse effects. “*” indicates p < 0.05, “**” indicates p < 0.01, “***” indicates p < 0.001.