Figure 1

Thy1⁺ cells are present in the Rag1^−/− epidermis.

(a) Flow cytometry of adult mouse epidermal cell suspension. Gating strategy to identify a Thy1⁺CD3⁻ population in both WT and Rag-deficient models is shown. (b). Histograms show relative expression intensity of CX₃CR1 and CD103 among Thy1.2⁺CD3⁻ cells. Representative data from n > 5 mice is shown for a–b. (c–d) Two-photon imaging of the epidermis of WT and Rag1^−/−Cx3cr1^gfp/+ mice. In green are the CX₃CR1-positive cells, scale 200 μm (c) and 20 μm (d). (e) Cell morphology analysis of CX₃CR1-positive cells from WT (black) and Rag1^−/− (grey) mice. Representative data of n = 3.