Figure 2

The epidermal Thy1⁺ population is heterogeneous.

(a) Flow cytometry of mouse epidermal cell suspension. Gating strategy and histograms to identify ICOS expression across the two populations: Thy1⁺, ILCs (CD3⁻CD2⁻, red) and ELCs (CD3⁻CD2⁺, blue) in both WT and Rag-deficient models is shown. (b) Histograms show relative expression intensity of CX₃CR1 among ELCs and ILCs from WT and Rag1^−/− mice. Representative data from n > 3 mice is shown a–b. (c) Identification of ELCs and ILCs in different mouse models. c. Absolute numbers of ELCs and ILCs in different mouse models. Representative data from n > 3 is shown, except for NSG where n = 2.