Figure 4
From: Identification of a novel lymphoid population in the murine epidermis
ELCs have a T cell progenitor-like phenotype.
(a) Flow cytometry of mouse epidermal cell suspensions with intracellular labelling for CD3. Histograms show the expression profile of intracellular CD3 for DETCs, ELCs and ILCs in several mouse models. (b) Plot shows the percentage of ELCs from WT (red), Rag1−/− (blue) and Nude (green) mice that express CD3 intracellularly. n = 5 for WT and Rag1−/− mice, n = 6 for Nude mice. (c) Gating scheme to identify pTα expressing cells from the mouse epidermis. The histograms and dot plots show the frequency of current or past pTα expression within the αβT-cell (red), DETC (blue), ELC (green) and ILC (orange) populations. Each data point represents an individual mouse. Data were obtained from n = 4 of Rosa26tdRFP/tdRFP control mice, Ptrcaicre/+ x Rosa26tdRFP/+ n = 10 on a WT background and n = 8 on a Rag1−/− background.
