Figure 6

ELCs are present before birth in both WT and Rag1^−/− mice.

(a) Flow cytometry of mouse epidermal cell suspensions. Gating strategies for DETC and Thy1⁺CD3⁻Vγ3⁻ populations are shown throughout mouse development (among CD45⁺Thy1⁺ cells). (b) Absolute numbers of both populations before birth, in newborns and in adult WT (•) and Rag1^−/− (■) mice. Representative data from n > 3. (c) Dot plots show the gating scheme revealing the heterogeneity of the Thy1⁺CD3⁻Vγ3⁻ population, which is mainly composed of ELCs, in the mouse epidermis. (d) Absolute numbers of ELCs and ILCs, before birth, in newborns and in adult WT (•) and Rag1^−/− (■) mice. Representative data from n > 3 (e) Purified neonatal ELCs were spun onto cytospin slides for Wright-Giemsa staining (Scale bar of 10 μm). Data are representative of 3 independent experiments. (f–g) Plots identify cells with a history of pTα-expression in E18.5 epidermis. The histograms and dot plot identify pTα-expressing cells within the DETC (blue) as well as ELC (green) populations. Each data point represents an individual embryo. Data were obtained from n = 36 embryos from 6 independent experiments of Ptrca^icre/+ x Rosa26^tdRFP/+ mice.