Figure 3
Functional evidence of isoflurane binding to the pore.
(a) Representative current traces for isoflurane inhibition of WT and two mutants: T237(6′)A; and A244(13′)T. The scale bars are 0.1 μA (vertical) and 30s (horizontal). (b) Agonist propylamine (PPA) concentration-response curves for WT and two mutants indicated in (a). The data were normalized to the maximum current and fit to the Hill equation (solid lines, n ≥ 5). (c) Isoflurane inhibition of WT and the two mutants indicated in (a). The data were normalized to the current at EC20 in the absence of isoflurane and fit to the Hill equation (solid lines, n ≥ 6). The data are reported as the mean ± SEM and error bars less than the symbol size are not visible.
