Figure 4
Isoflurane binding stabilized the closed channel.
(a) A representative trace showing that pre-incubation with isoflurane (red arrows) slowed the onset rate and increased the maximum inhibition. The vertical and horizontal scale bars represent 20 μA and 30 seconds, representatively. (b) The onset rate of the channel current can be fitted to a single exponential decay function, I = Imax (1−e(−t/τ)). The fitting results for the onset rate τ and Imax for channels pre-incubated with isoflurane (red line) are 1.73 ± 0.04 s and −0.33 ± 0.02 μA, respectively; and 1.25 ± 0.05 s and −0.41 ± 0.03 μA, respectively, for channels without isoflurane pre-incubation (green line). The fitting parameters are expressed as mean ± SEM (n = 15).
