Figure 1
From: Nano-architecture of gustatory chemosensory bristles and trachea in Drosophila wings
Distribution of gustatory sensilla and calcium imaging in Drosophila wings.
(A) The photograph shows a wing subset corresponding to the anterior wing margin. The stout bristles are mechanosensilla and the thin hairs (red arrows) are chemosensilla that shelter gustatory neurons. The dark line along the anterior margin is the vein of the anterior wing margin that contains haemolymph, trachea and the nerve made of bundles of axons of the mechanosensory and chemosensory (gustatory) neurons (see the schematic of a cross section in the box at the right). (B) The photographs display calcium imaging by a fluorescent probe. The gustatory sensilla light up after denatonium (1 mM) or glucose (50 mM) binding to their respective receptors. The wing corresponds to the F1 progeny of a cross between homozygous UAS-GCamp and heterozygous Tub-GAL4 flies. The photographs (1 to 4) represent successive sequences over a total period of 20 seconds after glucose stimulation (1 is the time 0 before induction). (C) Time course of the fluorescence after denatonium (1 mM) or glucose (50 mM) stimulation. The graphs represent the average of the determination (delta F/F0) for 10 wings and 3 sensilla per wing (n = 30, mean + /−SE, p < 0.0005 between the 15 and 30 sec., Student’s t-test).
