Figure 3: Cytotoxic activity of MUSC 136T extract against human cancer cell lines.

The measurement of cell viability was done using MTT assay. The graphs show cytotoxic effect of MUSC 136T extract against (a) HCT-116, (b) Ca Ski, (c) A549, (d) HT-29. All data are expressed as mean ± standard deviation (n = 4) and analyzed using one-way analysis of variance (ANOVA). A difference was considered statistically significant when p ≤ 0.05. (e) Morphological studies show that after treatment with the extract, cells shrunk to smaller rounder cells and detached from the surface.