Table 1 Differentiation characteristics of strain MUSC 136T and type strains of phylogenetically closely related species of the genus Streptomyces.

From: Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines

Characteristic

1

2

3

4

Morphology (on ISP 2):

    

Color of aerial mycelium

Yellow white

Yellowish white

Yellowish white

Light yellow

Color of substrate mycelium

Pale yellow

Strong yellow

Yellowish gray

Brilliant greenish yellow

Growth at:

    

24 °C

(+)

(+)

(+)

(+)

40 °C

(+)

+

+

pH 6

(+)

(+)

(+)

+

4% NaCl

(+)

+

(+)

Catalase

+

+

+

+

Hemolytic

Hydrolysis of:

    

Casein (protease)

+

+

Tributyrin (lipase)

+

+

+

Starch (amylolytic)

+

+

+

+

Carboxymethylcellulose (cellulase)

+

+

+

Xylan (xylanase)

+

Carbon source utilization:

    

Sucrose

+

+

+

Stachyose

+

+

+

D-raffinose

+

+

α-D-lactose

+

+

+

D-melibiose

+

β-methyl-D-glucoside

+

D-salicin

+

N-acetyl-b-D-mannosamine

+

+

N-acetyl-neuraminic acid

+

+

+

D-fucose

+

+

+

L-fucose

+

+

+

L-rhamnose

+

D-sorbitol

+

+

Myo-inositol

+

+

D-glucose-6-PO4

+

+

+

D-aspartic acid

+

+

+

D-serine

+

+

Chemical sensitivity assays:

    

Troleandomycin

+

+

+

Vancomycin

+

+

+

Fusidic acid

+

+

+

Rifamycin RV

+

+

+

Minocycline

+

+

+

Lincomycin

+

+

+

Niaproof 4

+

+

+

  1. Strains: 1, Streptomyces malaysiense sp. nov. MUSC 136T; 2, Streptomyces misionensis NBRC 13063T; 3, Streptomyces phaeoluteichromatogenes DSM 41898T; 4, Streptomyces rutgersensis NBRC 12819T. All data were obtained concurrently in this study. +, Positive; −, negative; (+), weak.
  2. All strains are positive for utilization of Dextrin, D-maltose, D-trehalose, D-cellobiose, Gentiobiose, D-turanose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, α-D-glucose, D-mannose, D-fructose, D-galactose, Inosine, D-mannitol, D-arabitol, Glycerol, D-fructose-6-PO4, Gelatin, Glycyl-L-proline, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-serine, Pectin, D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, D-glucuronic acid, Glucuronamide, Methyl pyruvate, L-lactic acid, L-malic acid, Bromo-succinic acid, Tween 40, γ-amino-butyric acid, α-hydroxy-butyric acid, β-hydroxy-D,L-butyric acid, α-keto-butyric acid, Acetoacetic acid, Propionic acid and Acetic acid. All strains are negative for assimilation of 3-methyl glucose.