Figure 1: Sequence alignment of NbCPIP2a, NbCPIP2b, NtCPIP2a, and NtCPIP2b proteins and phylogenetic analysis of NbCPIP2a-like proteins.

(A) Abbreviations for gene names are as follows: Nb2a (NbCPIP2a), Nb2b (NbCPIP2b), Nt2a (NtCPIP2a), and Nt2b (NtCPIP2b). The red line and blue line indicate the conserved DnaJ domain and the DnaJ C terminal domain, respectively. Aligned sequences were visualized with the Megalign program. (B) Phylogenetic relationship of NbCPIP2a-like proteins identified from plants for which draft genome sequences are available. The phylogenetic tree was constructed by protein sequences of NbCPIP2a-like proteins from diverse plants. NbCPIP2a-like proteins were identified by blast search in the Phytozome database. For simplicity, only plant species names instead of protein names are indicated in the phylogenetic tree. The phylogenetic tree was generated by the neighbor-joining method with 1,000 bootstrap replicates and Kimura 2-parameter distance using the MEGA6 program55.