Figure 4: Transient silencing of NbCPIP2a and NbCPIP2b in response to PVX infection. | Scientific Reports

Figure 4: Transient silencing of NbCPIP2a and NbCPIP2b in response to PVX infection.

From: Two homologous host proteins interact with potato virus X RNAs and CPs and affect viral replication and movement

Figure 4

(A) Schemes of silencing vectors. (B) Gene-silencing activities induced by RNAi vectors. (C) Intercellular movement of GFP-tagged PVX in transiently silenced N. benthamiana leaves. (D) PVX viral RNA replication in the extracted protoplasts. (E) Long-distance movement of GFP-tagged PVX in the transiently silenced N. benthamiana leaves. NC = negative control (leaves were not silenced and were not inoculated with PVX). PC = positive control (leaves were not silenced but were inoculated with PVX). 1st = the first leaf of the top. 5th = the fifth leaf from the top. NbCPIP2a-i or NbCPIP2b-i (leaves were infiltrated with the silencing vectors of NbCPIP2a or NbCPIP2b and were inoculated with PVX).

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