Figure 2: Characterization of the ApPAH gene.

(a) Schematic overview of ApPAH gene structure. Exons, 5′ and 3′ untranslated region (UTR), and introns are represented with grey boxes, white boxes, and solid lines, respectively. Arrowheads indicate the translation start site (ATG) and stop codon (TGA). Genomic coordinates refer to the positions on the scaffold GL350506 available from AphidBase38. (b) Nucleotide and amino acid sequences of the ApPAH gene. ApPAH comprises a 1,359 bp CDS encoding 452 amino acid residues, indicated as single-letters above the nucleotide sequence. Conserved binding domains are highlighted in different colors. The stop codon is marked with an asterisk. Underlined bold letters and red letters indicate the polyadenylation signal (AATAAA) and the sequence targeted by the dsApPAH, respectively.