Articles in 2017

iTango confers access to neuromodulation-sensitive subsets of neurons in a functionally defined and temporally controlled manner. The tool allows for manipulating the subset of neurons that are activated by dopamine during a behavior of interest.

This paper describes an in vitro method to generate human T cells from hematopoietic stem and progenitor cells (HSPCs). It should be useful for both basic and applied studies using T cells.

Deep brain imaging with three-photon microscopy and a swim from the US to China.

Thin slices through nuclei provide an unbiased view of the complex 3D organization of mammalian genomes.

Cryptic, widespread DNA damage is commonly interpreted as sequence variation.

A new approach measures nanoscale distances based on magnetic resonance tuning.

Bessel focus scanning and stereoscopy speed up volumetric brain imaging.

Advanced signal processing techniques enable the genome-wide analysis of methylation in native DNA using nanopore sequencing.

Trans-synaptic tracing that starts from a single neuron provides a detailed view of that neuron's inputs.

Many possibilities for parsing cancer emerge when labs combine gene editing and screens. And RNAi retains its spot in the menu of options.

Automated tracing algorithms can extract neuronal morphology from fluorescent or brightfield images. UltraTracer scales up the capability of existing tracing algorithms to handle datasets of ever-increasing size.

This Review introduces the fundamental considerations for building a light sheet microscope, describes the pros and cons associated with available implementations, and offers practical advice for users.