Articles in 2010

Array tomography opens the door to the large-scale exploration of molecular diversity of individual brain synapses.

RNA molecules can both induce pluripotency and direct differentiation.

A predictive model of intercellular metabolic interactions in the human brain is reported.

Three groups achieve room-temperature, single-molecule detection of nonfluorescent, photon-absorbing compounds.

A team of researchers applied a 'discovery single-particle profiling' experimental strategy to visualize the assembly of the ribosome via time-resolved electron microscopy.

Potent and easily produced custom zinc-finger nucleases will be game-changers for the field, at a time when the field itself is changing.

Microarrays made from naturally expressed glycolipids help winnow function from heterogeneous glycan structures.

Stabilization with a suction window permits in vivo imaging of the mouse lung vasculature with video-rate two-photon microscopy.

The low cost of short-read sequencing has motivated the development of de novo assemblies from only short-read data; impressively, assemblies for large mammalian genomes are now available. However, this is still a developing field, and these de novo assemblies have many artifacts, as do all de novo assemblies.

Small conditional RNAs prove their mettle in multiplexed mRNA imaging and show promise as potential cancer therapeutics.

Fluorescent proteins can be located in electron micrographs.

Advances in microfluidics and imaging, combined with some high-profile studies, are increasing interest in whole-organism screening.

A microfluidic embryo-trap array for large-scale end-on imaging of Drosophila melanogaster embryos permits quantitative analysis of dorsoventral gradients during development.

The Network-Free Stochastic Simulator (NFsim) allows the representation of complex biological systems as rule-based models and facilitates coarse-graining of the reaction mechanisms.

The payoffs for efficient cargo delivery into living cells make the development of better methods worthwhile.

Targeted analysis of proteins on a broad scale with mass spectrometry is becoming a reality.