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In this protocol update, the authors improve on their previous protocol for genome engineering of mammalian cultured cells with CRISPR–Cas9 to generate homozygous knock-ins of fluorescent tags into endogenous genes, to increase both efficiency and throughput.
Native mass spectrometry can be combined with lipidomic experiments to determine the structural and functional lipids of receptor and transporter assemblies. This protocol describes how to use initial native mass spectrometry results to guide experimental design.
Developing optimal nanozymes requires standardized methods for measuring their catalytic activity and reaction kinetics. This protocol integrates enzyme based Michaelis–Menten kinetics with measured physical properties and computational methods.
The controlled positioning and stoichiometry of aptamers on tetrahedral DNA frameworks enables the synthesis of targeted probes for the capture of circulating tumor cells from blood samples.
