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In this Tools of the Trade article, Qisi Zhang and Longlong Si describe a method for generating live attenuated vaccines that involves targeting viruses to the host cells’ protein degradation machinery, which attenuates the virus within the host and also enhances viral antigen presentation.
In this Tools of the Trade article, Sina Djafari Rouhani and Mohammad Rashidian describe CD45-PET for whole-body imaging of immune activity and dynamics.
In this Tools of the Trade article, Sofia Bustamante Eguiguren (from the Dushek and van der Merwe labs) describes CombiCells, which can be customized to express any combination and concentration of ligands to study receptor–ligands interactions.
In this Tools of the Trade article, Takamasa Kudo (in the Aviv Regev lab, in collaboration with Eric Lubeck) describes how PerturbView enables robust visualization of many thousands of genetic perturbations.
In this Tools of the Trade article, Sarah Al Abdullatif describes a method to measure — in a physiological context — mechanical forces transmitted between a T cell receptor and its ligand.
In this Tools of the Trade article, Yishay Pinto describes a tool, called Phanta, that profiles phages alongside their bacterial hosts in microbiome analysis.
In this Tools of the Trade article, Mayar Allam and Ahmet Coskun describe how they combined spatial metabolomics and proteomics profiling — in a framework they call scSpaMet — to explore, at the single-cell level, how metabolic profiles vary by location and in disease.
In this Tools of the Trade article, Camilla Engblom and colleagues describe their elegant technique ‘Spatial VDJ’ to detect and map antigen receptor sequences in human tissue sections.
In this Tools of the Trade article, Sandra Nakandakari-Higa (of the Gabriel Victora lab) describes the latest version of their LIPSTIC technique for tracking diverse cell–cell interactions in vivo.
Sizun Jiang (while in the Garry Nolan lab) describes a method termed PANINI that allows simultaneous detection of nucleic acids at low copy numbers and protein markers in intact tissues, offering valuable insights into virus–immune system interactions and beyond.
In this Tools of the Trade article, Giulia Adriani and Andrea Pavesi describe a new microfluidic device that supports the generation of vascularized 3D tumour models.
In this Tools of the Trade article, Zülal Cibir and Matthias Gunzer introduce their multi-lens array microscope called ComplexEye that allows high-throughput analysis of cell migration.
In this Tools of the Trade article, Leanne Arakkal (of the Ronald Germain group) describes a technique for highly multiplexed imaging of intact tissues.
In this Tools of the Trade article, Lisa Wagar (while in the Mark Davis lab) developed an in vitro immune organoid system from primary human lymphoid tissue that recapitulates productive adaptive immune responses.
In this Tools of the Trade article, Yury Goltsev and Garry Nolan describe a multiplexed tissue imaging technique called CODEX that enables rapid tissue staining with multiple DNA-barcoded antibodies.
In this Tools of the Trade article, Christa Haase (at the Charles Lin lab) describes a technique called Image-seq that enables image-guided cell isolation from a specific, intact tissue location for subsequent single-cell gene expression analysis.
In this Tools of the Trade article, Xiaoyun Dai (of the Sidi Chen lab) developed a CRISPR-based platform termed CLASH to generate a large pool of precisely gene-modified CAR T cells, from which the most desirable variants could be selected.
