Microbial natural products are an important source for antibiotic discovery, however, their efficient dereplication remains challenging. Here, the authors develop an analytical pipeline, nanoRAPIDS, to prioritize low abundance bioactive compounds at nanoscale, by integrating the bioassay of interest, automated mass spectrometry identification and GNPS-based dereplication, resulting in the discovery of saquayamycin N from Streptomyces sp. MBT84.
- Isabel Nuñez Santiago
- Nataliia V. Machushynets
- Gilles P. van Wezel
