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Nature Protocols (4)
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Showing 1–5 of 5 results

Advanced filters: Author: Christopher S Theile Clear advanced filters

Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry

Martin D Witte
Christopher S Theile
Hidde L Ploegh
Protocols29 Aug 2013
Nature Protocols

Volume: 8, P: 1808-1819
Expanding RNAi therapeutics to extrahepatic tissues with lipophilic conjugates

Lipophilic siRNA conjugates exert therapeutic activity in the mouse CNS.

Kirk M. Brown
Jayaprakash K. Nair
Vasant Jadhav
Research02 Jun 2022
Nature Biotechnology

Volume: 40, P: 1500-1508
Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Sortase A can be used for the site-specific modification of proteins. This protocol describes its immobilization on Sepharose beads, allowing for larger-scale continuous flow reactions or easy separation from the enzyme in batch reactions.

Martin D Witte
Tongfei Wu
Hidde L Ploegh
Protocols26 Feb 2015
Nature Protocols

Volume: 10, P: 508-516
Site-specific N-terminal labeling of proteins using sortase-mediated reactions

Christopher S Theile
Martin D Witte
Carla P Guimaraes
Protocols29 Aug 2013
Nature Protocols

Volume: 8, P: 1800-1807
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Carla P Guimaraes
Martin D Witte
Hidde L Ploegh
Protocols29 Aug 2013
Nature Protocols

Volume: 8, P: 1787-1799