Aminoglycoside resistance is predominantly mediated by enzymatic modification, wherein resistance enzymes typically bind the antibiotics in a low-energy conformation that closely mimics their ribosome-bound state. Here, the authors reveal that AAC(3)-Ia and AAC(3)-XIa deviate from this strategy by binding aminoglycosides in a unique, strained conformation, suggesting alternative mechanisms for antibiotic resistance.
- Mark Hemmings
- Michał Zieliński
- Albert M. Berghuis
