Nature Search

Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators

Mass cytometry can measure up to 34 markers on cells, but samples cannot be multiplexed. Bodenmiller et al. use metal ion tags to facilitate multiplexed mass cytometry analysis of human blood cell samples treated with 27 kinase inhibitors and 12 stimuli across a range of dosages and time points.

Bernd Bodenmiller
Eli R Zunder
Garry P Nolan
Research19 Aug 2012
Nature Biotechnology

Volume: 30, P: 858-867
A single-cell mass cytometry-based atlas of the developing mouse brain

In recent years, valuable mRNA-based developmental atlases of the mouse brain have been made available. Here, the authors use single-cell mass cytometry to build a protein-based single-cell profiling of the developing embryonic and postnatal mouse brain.

Amy L. Van Deusen
Sushanth Kumar
Eli R. Zunder
Research18 Dec 2024
Nature Neuroscience

Volume: 28, P: 174-188
Highly multiplexed simultaneous detection of RNAs and proteins in single cells

PLAYR (proximity ligation assay for RNA) enables highly multiplexed transcript quantification in combination with protein marker detection in single cells using flow or mass cytometry.

Andreas P Frei
Felice-Alessio Bava
Pier Federico Gherardini
Research25 Jan 2016
Nature Methods

Volume: 13, P: 269-275
Early reprogramming regulators identified by prospective isolation and mass cytometry

Identification of transient early induced pluripotency reprogramming intermediates allows for mechanistic insight into the reprogramming process.

Ernesto Lujan
Eli R. Zunder
Marius Wernig
Research01 Apr 2015
Nature

Volume: 521, P: 352-356
A developmental atlas of somatosensory diversification and maturation in the dorsal root ganglia by single-cell mass cytometry

Somatosensory neurons detect pain, temperature and touch. Keeler et al. constructed a single-cell, protein-level atlas of nearly 3 million cells from the mouse dorsal root ganglia, covering 13 days of embryonic and postnatal development.

Austin B. Keeler
Amy L. Van Deusen
Eli R. Zunder
Research27 Oct 2022
Nature Neuroscience

Volume: 25, P: 1543-1558
FLOW-MAP: a graph-based, force-directed layout algorithm for trajectory mapping in single-cell time course datasets

This protocol describes FLOW-MAP, a graph-based algorithm for visualizing cellular trajectories in single-cell time course datasets. The R package can be operated via its GUI or using text-based commands.

Melissa E. Ko
Corey M. Williams
Eli R. Zunder
Protocols13 Jan 2020
Nature Protocols

Volume: 15, P: 398-420
Direct comparison of mass cytometry and single-cell RNA sequencing of human peripheral blood mononuclear cells

Emily Y. Su
Kristen Fread
Patrick Cahan
ResearchOpen Access30 May 2024
Scientific Data

Volume: 11, P: 1-7
Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm

This protocol describes how to use mass-tag cell barcoding to label individual cell samples with unique combinatorial barcodes. The samples can then be pooled for processing and measurement on a mass cytometer.

Eli R Zunder
Rachel Finck
Garry P Nolan
Protocols22 Jan 2015
Nature Protocols

Volume: 10, P: 316-333

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