Nature Search

Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation

The authors demonstrate how avian H5N1 influenza A virus polymerase interacts with human ANP32B to facilitate the replication of the viral genetic information in mammalian hosts, revealing insights into cross-species transmission.

Ecco Staller
Loïc Carrique
Ervin Fodor
ResearchOpen Access15 May 2024
Nature Communications

Volume: 15, P: 1-10
Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies

Influenza viruses carry their own RNAdependent RNA-polymerase that is highly conserved and a promising anti-viral target. Combining functional and structural data, Keown et al. characterise the inhibitory effect of nanobodies on 1918 pandemic H1N1 influenza strain polymerase complex and identify sensitive sites interfering with polymerase activity in vitro.

Jeremy R. Keown
Zihan Zhu
Jonathan M. Grimes
ResearchOpen Access11 Jan 2022
Nature Communications

Volume: 13, P: 1-11
Host ANP32A mediates the assembly of the influenza virus replicase

Structural and biochemical studies of influenza virus RNA polymerase in complex with host acidic nuclear phosphoprotein 32 (ANP32) show how ANP32-mediated polymerase dimerization enables the replication of influenza viral RNA in a host-dependent manner.

Loïc Carrique
Haitian Fan
Jonathan M. Grimes
Research18 Nov 2020
Nature

Volume: 587, P: 638-643
Publisher Correction: Structural insights into RNA polymerases of negative-sense RNA viruses

Aartjan J. W. te Velthuis
Jonathan M. Grimes
Ervin Fodor
Amendments and Corrections02 Feb 2021
Nature Reviews Microbiology

Volume: 19, P: 220
Differential use of importin-α isoforms governs cell tropism and host adaptation of influenza virus

Interspecies transmission of influenza A viruses requires adaptation of the viral polymerase to the host importin-α. Here, the polymerase subunit PB2 and the nucleoprotein of avian viruses are found to require importin-α3, whereas mammalian viruses are shown to require importin-α7.

Gülsah Gabriel
Karin Klingel
Hans-Dieter Klenk
ResearchOpen Access18 Jan 2011
Nature Communications

Volume: 2, P: 1-7
Crystal structure of the RNA-dependent RNA polymerase from influenza C virus

The X-ray crystal structure of influenza C virus polymerase, captured in a closed, pre-activation confirmation, is solved at 3.9 Å resolution; comparison with previous RNA-bound structures reveals large conformational changes associated with RNA binding and activation, and illustrates the notable flexibility of the influenza virus RNA polymerase.

Narin Hengrung
Kamel El Omari
Ervin Fodor
Research26 Oct 2015
Nature

Volume: 527, P: 114-117
Influenza virus RNA polymerase: insights into the mechanisms of viral RNA synthesis

In this Review, te Velthuis and Fodor detail the recently obtained high-resolution structures of the influenza virus RNA polymerase and the insights that have been gained into the mechanisms of viral transcription and replication. They also discuss how these structural data could help to identify novel antiviral targets.

Aartjan J. W. te Velthuis
Ervin Fodor
Reviews11 Jul 2016
Nature Reviews Microbiology

Volume: 14, P: 479-493
Erratum: Conserved and host-specific features of influenza virion architecture

Edward C. Hutchinson
Philip D. Charles
Ervin Fodor
Amendments and Corrections17 Feb 2015
Nature Communications

Volume: 6, P: 1
Structures of influenza A virus RNA polymerase offer insight into viral genome replication

Structures of RNA polymerase of human and avian influenza A viruses reveal that the interface of the RNA polymerase dimer is required to initiate viral RNA synthesis in viral genome replication.

Haitian Fan
Alexander P. Walker
Ervin Fodor
Research04 Sept 2019
Nature

Volume: 573, P: 287-290
Conserved and host-specific features of influenza virion architecture

Influenza-infected cells produce viral particles that incorporate a mixture of viral and host proteins. Here the authors analyse by mass spectrometry the protein composition of such particles and show that the host species determines certain characteristics of an otherwise conserved architecture.

Edward C. Hutchinson
Philip D. Charles
Ervin Fodor
Research16 Sept 2014
Nature Communications

Volume: 5, P: 1-11
Structural insights into RNA polymerases of negative-sense RNA viruses

In this Review, Fodor and colleagues discuss recent structural insights into the RNA polymerases of negative-sense RNA viruses and their use to identify novel targets for the development of antiviral agents.

Aartjan J. W. te Velthuis
Jonathan M. Grimes
Ervin Fodor
Reviews25 Jan 2021
Nature Reviews Microbiology

Volume: 19, P: 303-318
An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization

Despite their essentiality, human ANP32A and ANP32B are redundant host factors for influenza virus genome replication. In this work, authors show that an influenza virus grown in cells lacking ANP32A and ANP32B evolved to use ANP32E. They explore the polymerase mutations that enable this, and demonstrate increased virulence in mice.

Carol M. Sheppard
Daniel H. Goldhill
Wendy S. Barclay
ResearchOpen Access10 Oct 2023
Nature Communications

Volume: 14, P: 1-16
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

Mutations to SpCas9 can improve fidelity and mitigate off-target effects. Here the authors generate ‘Blackjack’ mutations that improve fidelity while retaining effectiveness with 21G-sgRNAs.

Péter István Kulcsár
András Tálas
Ervin Welker
ResearchOpen Access06 Mar 2020
Nature Communications

Volume: 11, P: 1-14
Mini viral RNAs act as innate immune agonists during influenza virus infection

Aberrant mini viral RNAs, which are produced by erroneous RNA polymerase activity during the replication of the viral RNA genome, act as the main agonists of RIG-I during influenza virus infection.

Aartjan J. W. te Velthuis
Joshua C. Long
Ervin Fodor
Research17 Sept 2018
Nature Microbiology

Volume: 3, P: 1234-1242
The structure of the influenza A virus genome

A combination of secondary structure probing and RNA crosslinking sequencing approaches sheds lights on the RNA conformations and the intra- and intersegment interactions of the genome inside influenza A virions.

Bernadeta Dadonaite
Brad Gilbertson
David L. V. Bauer
Research22 Jul 2019
Nature Microbiology

Volume: 4, P: 1781-1789
The role and assembly mechanism of nucleoprotein in influenza A virus ribonucleoprotein complexes

The nucleoprotein of negative-strand RNA viruses forms a part of the ribonucleoprotein complex. Here Turrell et al.show that nucleoprotein does not regulate the initiation and termination of transcription and replication by the viral RNA polymerase, suggesting that nucleoprotein instead is an elongation factor.

Lauren Turrell
Jon W. Lyall
Frank T. Vreede
Research12 Mar 2013
Nature Communications

Volume: 4, P: 1-11
Crystal structure of an avian influenza polymerase PA_N reveals an endonuclease active site

This paper reports the crystal structure of the amino terminus of the PA subunit of the influenza RNA polymerase, and provides evidence that it has endonuclease activity.

Puwei Yuan
Mark Bartlam
Yingfang Liu
Research04 Feb 2009
Nature

Volume: 458, P: 909-913
The G127V variant of the prion protein interferes with dimer formation in vitro but not in cellulo

Sudheer Babu Sangeetham
Anna Dorothee Engelke
Ervin Welker
ResearchOpen Access04 Feb 2021
Scientific Reports

Volume: 11, P: 1-13
The role of the priming loop in influenza A virus RNA synthesis

Influenza A virus polymerase has a β-hairpin in the thumb subdomain, which is shown to be essential for the initiation of viral replication, but auxiliary for other replicative steps and viral transcription.

Aartjan J. W. te Velthuis
Nicole C. Robb
Ervin Fodor
Research21 Mar 2016
Nature Microbiology

Volume: 1, P: 1-7
The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells

Antal Nyeste
Claudia Stincardini
Ervin Welker
ResearchOpen Access07 Nov 2016
Scientific Reports

Volume: 6, P: 1-12

Search

Filter By:

Search

Quick links