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Showing 1–2 of 2 results
Advanced filters: Author: Florian P. Weissenboeck Clear advanced filters

  • Analogues of mRNA 5′ caps containing a photo-cleavable group have now been developed. These so-called FlashCaps can be used for routine in vitro transcription to make long mRNAs containing a cap. In cells, the capped mRNAs are translationally muted; however, upon irradiation by light, the photo-cleavable group is removed without leaving any remaining modification and mRNA is then translated into the corresponding protein.

    • Nils Klöcker
    • Florian P. Weissenboeck
    • Andrea Rentmeister
    ResearchOpen Access
    Nature Chemistry
    Volume: 14, P: 905-913

  • Translation of mRNA into protein is a fundamental process and tightly controlled during development, however, experimentally controlling the mRNA translation remains challenging. Here, the authors demonstrate the translation of FlashCap-eGFP-mRNA can be triggered via light-mediated activation in zebrafish embryos with spatiotemporal control.

    • Florian P. Weissenboeck
    • Melissa Pieper
    • Andrea Rentmeister
    ResearchOpen Access
    Communications Chemistry
    Volume: 8, P: 1-8