Nature Search

A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.

Sarah A. Nordeen
Kasper R. Andersen
Thomas U. Schwartz
ResearchOpen Access02 Dec 2020
Nature Communications

Volume: 11, P: 1-13
Regulation of monoubiquitinated PCNA by DUB autocleavage

Tony T. Huang
Sebastian M.B. Nijman
Alan D. D'Andrea
Research12 Mar 2006
Nature Cell Biology

Volume: 8, P: 341-347
In vivo discovery of immunotherapy targets in the tumour microenvironment

A short hairpin RNA screen to identify genes that modify the action of tumour-infiltrating CD8 T cells in tumour-bearing mice pinpointed the phosphatase subunit Ppp2r2d as a new target for tumour therapy; knockdown of Ppp2r2d in T cells enabled their accumulation in tumours and significantly delayed tumour growth.

Penghui Zhou
Donald R. Shaffer
Kai W. Wucherpfennig
Research29 Jan 2014
Nature

Volume: 506, P: 52-57
Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism

The CARD-only protein INCA inhibits inflammasome assembly by capping caspase-1 CARD oligomers and preventing their further polymerization.

Alvin Lu
Yang Li
Hao Wu
Research04 Apr 2016
Nature Structural & Molecular Biology

Volume: 23, P: 416-425
LMP2 expression and proteasome activity in NOD mice

Benedikt M. Kessler
Ana-Maria Lennon-Duménil
Hidde L. Ploegh
Correspondence01 Oct 2000
Nature Medicine

Volume: 6, P: 1064
Dendritic Cells: Biology and Clinical Applications

Hidde L. Ploegh
Books & Arts01 Sept 1999
Nature Medicine

Volume: 5, P: 979
Upregulation of the CLIP self peptide on mature dendritic cells antagonizes T helper type 1 polarization

Till A Röhn
Marianne Boes
Harald Kropshofer
Research22 Aug 2004
Nature Immunology

Volume: 5, P: 909-918
A CREB3–ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens

In an insertional mutagenesis screen, Sabatini and colleagues identify the small G protein ARF4 as a mediator of cell death in response to brefeldin A (BFA) treatment. BFA-induced Golgi stress upregulates ARF4, and loss of ARF protects against propagation of pathogens known to induce Golgi fragmentation.

Jan H. Reiling
Andrew J. Olive
David M. Sabatini
Research03 Nov 2013
Nature Cell Biology

Volume: 15, P: 1473-1485
Global gene disruption in human cells to assign genes to phenotypes by deep sequencing

Carette et al. use a retroviral gene-trap vector to disrupt and tag >98% of all genes in a haploid human cancer cell line. Cells selected for a phenotype of interest are pooled and characterized by parallel DNA sequencing to circumvent time- and labor-intensive screening of individual clones.

Jan E Carette
Carla P Guimaraes
Thijn R Brummelkamp
Research29 May 2011
Nature Biotechnology

Volume: 29, P: 542-546
An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model

Jasdave S. Chahal
Tao Fang
Hidde L. Ploegh
ResearchOpen Access21 Mar 2017
Scientific Reports

Volume: 7, P: 1-9
Crystal structure of a substrate-engaged SecY protein-translocation channel

The crystal structure of a substrate-engaged SecY channel and the SecA ATPase, which provides molecular insight into the process of protein translocation across membranes.

Long Li
Eunyong Park
Tom A. Rapoport
Research07 Mar 2016
Nature

Volume: 531, P: 395-399
A nanobody that binds to the backside of the ubiquitin conjugating enzyme Ube2G2 differentially affects interactions with its partner E3 Ligases

A nanobody that binds to the backside of the ubiquitin conjugating enzyme Ube2G2 differentially affects interactions with its partner E3 Ligases

Pavana Suresh
Charlotte Wijne
Hidde Ploegh
ResearchOpen Access16 Apr 2025
Communications Biology

Volume: 8, P: 1-15
CEACAM1 regulates TIM-3-mediated tolerance and exhaustion

CEACAM1 functions as a novel heterophilic ligand for TIM-3 and is necessary for TIM-3-mediated tolerance, which has marked consequences for inflammation, infection and cancer.

Yu-Hwa Huang
Chen Zhu
Richard S. Blumberg
Research26 Oct 2014
Nature

Volume: 517, P: 386-390
Translating cell biology in vitro to immunity in vivo

Marianne Boes
Hidde L. Ploegh
Reviews08 Jul 2004
Nature

Volume: 430, P: 264-271
Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Sortase A can be used for the site-specific modification of proteins. This protocol describes its immobilization on Sepharose beads, allowing for larger-scale continuous flow reactions or easy separation from the enzyme in batch reactions.

Martin D Witte
Tongfei Wu
Hidde L Ploegh
Protocols26 Feb 2015
Nature Protocols

Volume: 10, P: 508-516
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Carla P Guimaraes
Martin D Witte
Hidde L Ploegh
Protocols29 Aug 2013
Nature Protocols

Volume: 8, P: 1787-1799
Site-specific N-terminal labeling of proteins using sortase-mediated reactions

Christopher S Theile
Martin D Witte
Carla P Guimaraes
Protocols29 Aug 2013
Nature Protocols

Volume: 8, P: 1800-1807
Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry

Martin D Witte
Christopher S Theile
Hidde L Ploegh
Protocols29 Aug 2013
Nature Protocols

Volume: 8, P: 1808-1819
T-cell-receptor gene therapy

Ton N. M. Schumacher
Reviews01 Jul 2002
Nature Reviews Immunology

Volume: 2, P: 512-519
The activity of myeloid cell-specific VHH immunotoxins is target-, epitope-, subset- and organ dependent

Christopher Bachran
Matthias Schröder
Lee Kim Swee
ResearchOpen Access20 Dec 2017
Scientific Reports

Volume: 7, P: 1-10
z-VAD-fmk inhibits peptide:N-glycanase and may result in ER stress

S Misaghi
G A Korbel
H L Ploegh
Correspondence08 Jul 2005
Cell Death & Differentiation

Volume: 13, P: 163-165
How B cells capture, process and present antigens: a crucial role for cell polarity

In this Review, the authors discuss the molecular mechanisms that enable B cells to internalize, process and present antigens on MHC class II molecules to CD4⁺T cells. They focus on the importance of cytoskeletal rearrangements and the establishment of polarity in B cells for these processes.

Maria-Isabel Yuseff
Paolo Pierobon
Ana-Maria Lennon-Duménil
Reviews25 Jun 2013
Nature Reviews Immunology

Volume: 13, P: 475-486