Tools for diversifying genomic DNA in mammalian cells have long relied on base editors making C to T or A to G substitutions. Here, authors use RNA-guided DNA polymerases (EvolvR) to evolve mammalian cells using all twelve substitutions and show that nickase fidelity affects EvolvR’s mutation rates.
- Juan E. Hurtado
- Adam J. Schieferecke
- John E. Dueber
