Cytochrome P450 monooxygenases can functionalize alkaloid scaffolds with striking stereo- and regioselectivity. Here, the authors integrate bioinformatics and enzyme discovery to identify P450 monooxygenases capable of selectively functionalizing anticancer alkaloid evodiamine, revealing their catalytic activities and potential application in diversifying pharmacologically important compounds.
- Brooke D. Kwan
- Truc Kim
- Thu-Thuy T. Dang
