Combining crystallographic data, MD simulations, mutagenesis, lipid transfer and liposome binding assays, Lu et al. determine the mechanism of PA transfer between mitochondrial membranes, mediated by the Ups1/Mdm35 protein family. In particular, they describe role of the conformational change of the omega loop or α2 loop of Ups1 in the Ups1 PA transfer activity.
- Jiuwei Lu
- Chun Chan
- Yujia Zhai