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Small proteins (<50 kDa) are difficult to resolve by cryo-EM due to low signal-to-noise ratios and alignment challenges. Here, authors engineered conformationally rigid antibody fragments (Rigid Fabs) enabling high-resolution cryo-EM structures of small (~20 kDa) proteins like KRAS.

Electrochemical ion pumping combines the advantages of conventional capacitive deionization and electrodialysis for effective ion separation. A mathematical model of the technique reveals aspects of ion transport that show fundamental differences from conventional capacitive deionization or electrodialysis.

The performance of inverted perovskite solar cells has been limited by non-radiative recombination at the perovskite surfaces. Here, authors employ phosphonic acids and piperazinium chloride for homogeneous passivation, achieving certified efficiency of 28.9% for 60 cm² perovskite-silicon tandems.

The extracellular matrix provides a critical scaffold for the epidermal layers of the skin. Here, He and Boraas et al. discover that the foundation for resilient skin healing in embryos lies in the supportive matrix beneath stem cells.

Maximum Intensity Projection is a common tool to represent 3D biological imaging data in a 2D space, but it creates artefacts. Here the authors develop Smooth Manifold Extraction, an ImageJ/Fiji plugin, to preserve local spatial relationships when extracting the content of a 3D volume to a 2D space.

Polymer thin films that emit and absorb circularly polarised light are promising in achieving important technological advances, but the origin of the large chiroptical effects in such films has remained elusive. Here the authors demonstrate that in non-aligned polymer thin films, large chiroptical effects are caused by magneto-electric coupling, not structural chirality as previously assumed.

The Rustenburg Layered Suite of the Bushveld Complex, South Africa, has long been regarded as a textbook result of fractional crystallization from a melt-dominated magma chamber. Here, the authors find that the Rustenburg Layered Suite can be derived from crustal assimilation by komatiitic magma to form magmatic mushes without requiring the existence of a magma chamber by using thermodynamic models.

This study reveals that graded expression of the transcription factor BRN3B fine-tunes the identity and function of melanopsin-expressing ipRGC subtypes in the retina. Altering BRN3B levels disrupts subtype-specific features and visual behaviour.

Recurring deep ocean oxygen minimum events occur in the Pacific Southern Ocean during the warm climate interval Marine Isotope Stage 11. They are linked with perturbed Antarctic Bottom Water formation caused by West Antarctic Ice Sheet retreat.

Fibroblasts represent a heterogenous cell population but how their differences reflect their plasticity and origin is not fully understood. Here, the authors map the origin and fate of a transient contractile fibroblast population that forms a niche supporting pubertal mammary gland branching and growth.

Amiad Pavlov, Heffler, et al. demonstrate that stress transmitted to the cardiomyocyte nucleus by the microtubule cage drives LMNA-associated cardiomyopathy and may represent a promising therapeutic target.

The performance of single-crystal perovskite solar cells has been limited by interfacial loss at the perovskite/charge transport layer. Here, authors fabricate an asymmetric substrate stack through space-confined inverse temperature crystallization, achieving maximum device efficiency of 24.32%.

Analysis of human Robertsonian chromosomes originating from 13, 14 and 21 reveal that they result from breaks at the SST1 macrosatellite DNA array and recombination between homologous sequences surrounding SST1.

CLEM-Reg automates the three-dimensional alignment of volume correlative light and electron microscopy datasets by leveraging probabilistic point cloud registration techniques for fast and accurate results across diverse datasets.

Defective neurotransmission is a hallmark of spinal muscular atrophy (SMA). Here, the authors show that local presynaptic Munc13-1synthesis is defective in SMA and that modification of the Munc13-1 mRNA rescues presynaptic architecture and excitability.

Viruses generally have compact genomes, resulting in many viral mRNAs with short 5’ untranslated regions. An antiviral complex exploits this feature of viral mRNAs to selectively inhibit viral protein synthesis and repress viral replication.

Scanning dielectric microscopy of nanocapillaries filled with water reveals that interfacial and strongly confined water exhibits a large in-plane dielectric constant and an in-plane conductivity approaching superionic values. 

MsbA mediates the transport of lipopolysaccharide across the inner membrane. Here, the authors show specific MsbA-lipid interactions can tune the selectivity for binding ATP over ADP and present five structures of MsbA with one bound to lipid.

McGeachan et al. observe oligomeric tau in synapses from individuals with progressive supranuclear palsy and provide evidence that tau pathology may spread through the brain via synapses.

Structural and functional characterization of the β-barrel assembly machinery complex in Bacteroidota reveals a distinct, seven-component complex with a large extracellular domain that may enable β-barrel–surface lipoprotein complex assembly.

The Antarctic Circumpolar Current (ACC) since the Last Interglacial is reconstructed, showing orbital-scale shifts in ACC position driven by eccentricity and precession, possibly counteracting the predicted southward shift due to global warming.

Seismological and geodetic data are used together with a machine learning earthquake catalogue to reconstruct magma migration before and during the 2025 volcano–tectonic crisis at Santorini volcano, indicating a coupling between Santorini and Kolumbo.

Systematic alteration of HIV-1 TAR RNA and quantitative determination of its propensity to bind to the Tat protein establish a key role role for a rare and short-lived RNA state in Tat-dependent transactivation in cells.

BamA is the catalytic core of the BAM complex, which inserts proteins into bacterial outer membranes. Here, the authors show that hinge flexibility between BamA’s β-barrel and POTRA domains is vital, demonstrating how evolution has fine-tuned the BamA sequence and structure for function.

Dense calcium imaging combined with co-registered high-resolution electron microscopy reconstruction of the brain of the same mouse provide a functional connectomics map of tens of thousands of neurons of a region of the primary cortex and higher visual areas.

Using single-cell and spatial transcriptomics on muscle samples from non-ischemic patients and patients with peripheral artery disease (PAD), Turiel et al. identify cellular and molecular changes in the muscle microenvironment during PAD, focusing on endothelial cell–macrophage interactions.

Exercise promotes recovery after stroke, but the underlying mechanisms are unclear. Here, the authors show that regulatory T cells enable exercise-induced repair by reducing neuronal hyperexcitability via interleukin-10, linking immunity to functional regeneration.

An optical slab waveguide with highly programmable nonlinear functionality is described, enabling the demonstration of versatile control over broadband second-harmonic generation across the spectral, spatial and spatio-spectral domains.

CryoEM snapshots of the nitrogenase-like DPOR protein complex captured during turnover reveal that asymmetric conformational changes, substrate recognition, and an interplay between copper and iron sulfur metalloclusters is required for electron transfer.

Adult stem cells often reside in specialized niches that they exit upon injury. Here they show that in Drosophila, after damage the gut-associated trachea produces the PDGF-VEGF-related ligand Pvf1, which acts as a guidance cue directing ISC migration toward injury sites.

Scaffolding protein ankyrin-B, implicated in cardiometabolic syndrome, enables mitochondrial fission and flexibility of nutrient utilization under energetic stress in skeletal muscle to support energy production and optimal exercise capacity.

An optical detection and analysis platform quantifies aggregate density, distribution and size in fluorescently labelled post-mortem human brain tissue.

Nguyen, Collier et al. find a mitochondria–lysosome inflammatory pathway regulated by the SUMO E3 ligase MAPL, which promotes vesicular mtDNA transport to lysosomes and subsequent gasdermin-dependent lysosomal permeabilization to activate pyroptosis.

Garnet-type LLZO electrolytes are considered among the most promising solid-state electrolytes for all-solid-state batteries; however, numerous challenges need to be addressed before they are integrated into a cell. By precipitating amorphous zirconium oxide onto grain boundaries, increased ionic conductivity is observed and dendrite growth is suppressed.

The microporous polymer membranes with the introduction of hydrophilic functional groups achieve high selectivity for monovalent/divalent ion separation in electrodialysis processes, providing an alternative approach to lithium extraction with easy scale-up.

The unusual electronic and optical properties of graphene are finding increasing applications for terahertz and mid-infrared plasmonics. Here, the authors show how monolayers of graphene separated by thin dielectric layers can act as tunable structures for plasmonic device platforms.

An Fe^III/V redox mechanism in Li₄FeSbO₆ on delithiation without Fe^IV or oxygen formation with resistance to aging, high operating potential and low voltage hysteresis is demonstrated, with implications for Fe-based high-voltage applications.

This study supports uncertainty-aware energy transition planning by identifying key uncertainty sources. In Puerto Rico, hurricane frequency and institutional inefficiency emerge as the two main drivers.

This work reveals how a regulatory domain in O-GlcNAc hydrolase (OGA) shapes enzyme flexibility and activity, uncovering mechanisms that help maintain O-GlcNAc balance in cells.

iExoKras^G12D are engineered exosomes for the delivery of siRNA targeting KRAS^G12D. Here the authors describe the results of a phase I trial of iExoKrasG12D in patients with metastatic pancreatic cancer, reporting safety and clinical activity, as well as immunological correlates informing on tumor immune microenvironment reprograming and future combination with immune checkpoint inhibitors.’

Shigella sonnei is divided into five lineages and most infections are caused by Lineage 3. Here, the authors investigate factors contributing to the epidemiological success of Lineage 3 through comparative genomics and virulence analyses in a zebrafish infection model.

A fresh approach to protein design that incorporates excited intermediate states enables precise control over the lifetime of protein interactions, with potential applications in cell-signalling modulation and in biosensors and synthetic circuits.

A reconstruction of temperatures in central and north Greenland from ad 1000 to 2011 shows that that the final decade of this period was on average 1.5 ± 0.4 °C warmer compared to pre-industrial temperatures, accompanied by increased meltwater run-off.