Investigations of reveromycin biosynthesis, including metabolite analysis, feeding experiments and in vitro tests of enzyme function, point to a two-step mechanism for formation of the central spiroacetal, in which RevG oxidizes a stable acyclic precursor and RevJ catalyzes the stereocontrolled cyclization.
- Shunji Takahashi
- Atsushi Toyoda
- Hiroyuki Osada
