It is challenging to purify and prepare large amount of methacrylated proteins from cells. Here, the authors develop genetic code expansion technology to site-specifically incorporate ε-N-Methacryllysine into proteins and identify the post-translational modification ε-N-methyl-ε-N-methacrylation.
- Tian-Yi Zhu
- Shi-Yi Chen
- Bing Yang
