Search

Regulation of the actin cytoskeleton is essential for blood-brain barrier integrity and immune cell transmigration. Here, the authors show that the ion channel K2P2.1 regulates this process by shielding key signaling lipids and modulating actin dynamics.

In atrial fibrillation patients, circulating biomarkers reflecting inflammation, myocardial injury, and coagulation improve prediction of stroke, heart failure, and bleeding, outperforming clinical scores and enhance risk stratification.”.

A lot of theoretical work on the Kondo effect has focused on spin 1/2 systems, but the characterization of a single-spin 1/2 atom or molecule in the weak coupling regime has been missing. Here, the authors close this gap with a scanning tunneling spectroscopy study of an organic radical on a gold surface.

Individual molecules are now deterministically trapped in few-femtosecond laser pulses. This molecular conveyer belt may become a useful tool for probing ultrafast molecular dynamics.

In a non-prespecified interim analysis of a phase 1 trial, autologous PRAME-directed TCR T cell therapy was safe and elicited durable responses in patients with recurrent and/or treatment-refractory PRAME⁺ advanced solid tumors, including melanoma and synovial sarcoma.

Time-resolved serial crystallography at XFELs reveals ultrafast photochemical reactions, but high laser densities can cause photodamage to biological samples. Here, the authors study the early K-intermediate in bacteriorhodopsin at high power, showing overall conformation remains robust over a wide range.

Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for sensing glyoxylate and glycolate, with applications in pathway engineering and environmental monitoring.

Plasmacytoid dendritic cells monitor the bone marrow for apoptotic megakaryocytes (MKs) and deliver IFNα to the MK niche, triggering local on-demand proliferation and maturation of MK progenitors.

Photopharmacology manipulates the biological activity of small molecules by light. Using an X-ray laser, the authors follow the release of the drug azo-combretastatin A4 from tubulin and the concomitant structural changes over nine orders of magnitude in time.

The need for redox balancing limits the array of fermentable substrate-product combinations in anaerobic microbe-based bioproduction. Here, the authors design and engineer an E. coli strain with new-to-nature aerobic fermentative metabolism that allows tightly controlled re-balanced fermentations.

IFITM proteins can inhibit several viruses, but effects on SARS-CoV-2 infection are not well understood. Here, the authors show that endogenous IFITMs support SARS-CoV-2 infection in different in vitro models by binding spike and enhancing virus entry.

Corporate procurement initiatives, such as RE100, can increase their impact on the energy transition by formulating ambitious interim targets and sourcing requirements, and by orchestrating corporate interests in countries with less ambitious renewable energy targets.

One-carbon substrates are attractive feedstocks for circular bioeconomy. Here, the authors design an erythrulose monophosphate (EuMP) cycle for formaldehyde assimilation, demonstrate the activity of the core reactions in E. coli, and show its integration with pathway reactions existed in pentose phosphate pathway and glycolysis.

Financing costs for renewable energy technologies have decreased substantially over the past 18 years, helping make renewables more cost competitive. Leveraging the effect of financial learning and continuing the policies that facilitated favourable financing conditions are key for greater renewable energy adoption in the future.

One picosecond after photoactivation, isomerized retinal pulls away from half of its numerous interactions with its binding pocket, and the excess of the photon energy is released through an anisotropic protein breathing motion in the direction of the extracellular space.

Using two-photon imaging in a transgenic mouse model of Alzheimer's disease, the authors find that microglia, the resident macrophages of the brain, surround neurons prior to nerve cell death. They also find that inactivation of the microglial chemokine receptor CX3CR1, which is critical in neuron-microglia communication, prevents neuron loss.

Solar-powered standalone systems drastically lower the cost of electrifying sub-Saharan Africa. Household electrification can be provided at 7c USD per person per day on average. To reflect inter- and intra-country variance, policymakers should consider electrification cost curves.

Multiomic factor analysis of blood multiomic data, including single-cell transcriptomics, for individuals with either acute or chronic coronary syndrome identifies immune cell signatures that correlate with treatment outcomes.

Though integrating functional organic materials with semiconductor nanostructures is attractive for 3D chip processing, realizing these hybrids remains a challenge. Here, the authors report an oxidative chemical vapor deposition-based process for designing novel 3D hybrid optoelectronic structures.

Although co-occurring species may differ widely in their response traits, coordinated functional trait shifts may emerge at the community level in response to environmental factors. Here, the authors use data from 150 grassland sites to identify a coordinated slow-fast strategy response to land-use intensification across above- and belowground taxa.

Metabolic distribution of fatty acids to organelles is an essential biological process for energy homeostasis. Here the authors report a fluorescent probe that allows multicolour visualisation of the intracellular distribution of exogenous fatty acids, metabolically incorporated as lipid components.

Renewable energy technologies are intended to contribute to electricity access both on grid and off grid in sub-Saharan Africa, yet their high cost of capital continues to hamper their growth. Using data from sub-Saharan Africa, Agutu et al. estimate the cost of capital at the country and technology level for electrification modes and find that the cost of capital is much higher than previously estimated in many cases.

Crystallographic ‘snapshots’ taken at intervals of femtoseconds to milliseconds after activation show how a light-activated sodium pump carries sodium ions across the cell membrane.

Charging costs are important for the diffusion of electric vehicles as required to decarbonize transport. Here, the authors show large variance of electrical vehicle charging costs across 30 European countries and charging options, suggesting different policy options to reduce charging costs.

Minimal photon fluxes (MINFLUX) has enabled molecule-scale resolution in fluorescence microscopy but this had not been shown in standard, broadly applicable microscopy platforms. Here the authors report a solution to allow normal fluorescence microscopy while also providing 1-3 nm 3D resolution.

Breakdown of vascular barriers is a major complication of inflammatory diseases. However, the mechanisms underlying platelet recruitment to inflammatory micro-environments remains unclear. Here, the authors identify haptotaxis as a key effector function of immune-responsive platelets

In this study, the authors generate a new mouse model that allows selective genetic targeting of microglial cells. Using this model, they show that elimination of TGF-β-activated kinase 1 (TAK1) specifically in microglial cells reduces pathology in a mouse model of multiple sclerosis by inhibiting NF-κB, ERK and JNK signaling pathways.

Lassé et al. show that genes involved in kidney organoid proteomic response to TNFα segregate a subset of individuals with poor outcomes in proteinuric kidney disease, demonstrating the relevance of kidney organoid modeling to human kidney disease.

A decrease in the cost of renewable energy is often attributed to technological learning. This study uses 18 years of data from 133 renewable energy projects in Germany, alongside practitioner interviews, to find that changing financing costs, not just technology, are responsible for a significant cost decrease.

Tumors can vary in both their control by immunosurveillance and their glycolytic activity. Bopp and colleagues demonstrate that highly glycolytic tumors acidify their microenvironment and use this to initiate a mechanism of localized immunosuppression.

Serial crystallography was developed for protein crystal data collection with X-ray free-electron lasers. Here the authors present several examples which show that serial crystallography using high-viscosity injectors can also be routinely employed for room-temperature data collection at synchrotrons.

Cervix and breast carcinomas are highly heterogeneous in their mechanical properties across scales. This heterogeneity provides the tumour with stability and room for cell motility.

Current efforts to establish synthetic carbon fixation in model heterotrophs rely on expression of heterologous enzymes. Here, the authors explore the presence and activity of a latent CO₂-assimilation pathway in E. coli based only on endogenous enzymes and a reversible decarboxylase.

Arterial macrophages develop from either yolk sac or bone marrow progenitors. Here, the author show that ageing-induced reduction of arterial macrophages is not replenished by bone marrow-derived cells, but under inflammatory conditions circulating monocytes are recruited to maintain homeostasis, while arterial macrophages of yolk sac origin carry out tissue repair.

Microglia progenitors seed the central nervous system from the yolk sac, but little is known about the origin of non-parenchymal macrophages. Prinz and colleagues demonstrate that these macrophages in the central nervous system are related to but distinct from microglia and are largely of embryonic origin.

Outer membrane protein HopQ binds human CEACAMs and serves as an adhesin for Helicobacter pylori.

Learning rates are a measure of reduction in costs of energy from technologies such as solar photovoltaics. These are often estimated internationally with all monetary figures converted to a single currency, often US dollars. Lilliestam et al. show that such conversions can significantly affect the learning rate estimates.

Dimethyl fumarate (DMF) is a therapy for multiple sclerosis (MS) with undetermined mechanism of action. Here the authors find that clinical response to DMF associates with decrease in IL-17-producing CD8⁺ T cells (Tc17), delineate molecular pathways involved, and show that DMF suppresses Tc17 pathogenicity in a mouse model of MS.

The function of memory CD8⁺ T cells is often believed to be directly correlated with their localization in tissues. Here the authors show that CD8⁺T cells with different proliferative and cytotoxic properties can be distinguished based on their expression of CX3CR1, independently of their tissue localization.