The authors developed a high-content, quantitative analysis of breast cancer tissues based on microfluidic staining and image processing, to characterize both HER2 overexpression and amplification at the cellular level. This study paves the way for evaluatation of intratumoral heterogeneity with unprecedented accuracy with standard staining methods such as immunofluorescence and FISH.
- Huu Tuan Nguyen
- Daniel Migliozzi
- Martin A. M. Gijs
