The gut microbiota offers an extensive resource of enzymes, however, many remain uncharacterized. Here, the authors apply an activity-based metaproteomics strategy using an activity-based cyclophellitol aziridine probe specific to α-galactosidases (AGAL), and identify and characterize several gut microbiota enzymes possessing AGAL activities.
- Jianbing Jiang
- Diana Czuchry
- Nan Li
