Fig. 1
From: Prospects of Carrier Screening of Aspartylglucosaminuria in Finland
Principle of solid-phase minisequencing: 1 = amplification with one biotinylated primer; 2 = affinity capture on a streptavidin-coated support; 3 = denaturation (removal of the unbiotinylated strand); 4 = annealing of a detection step primer and elongation with one labeled nucleotide; 5 = denaturation; 6 = eluted radioactivity measured in a liquid scintillation counter.
