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Identification of human APC10/Doc1 as a subunit of anaphase promoting complex

Oncogene volume 18, pages 5131–5137 (1999)Cite this article

Abstract

Anaphase-promoting complex or cyclosome (APC) is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B. Identification of the subunits of multiprotein complex APC in several species revealed the highly conserved composition of APC from yeast to human. It has been reported, however, that vertebrate APC is composed of at least eight subunits, APC1 to APC8, while budding yeast APC is constituted of at least 12 components, Apc1 to Apc13. It has not yet been clearly understood whether additional components found in budding yeast, Apc9 to Apc13, are actually composed of mammalian APC. Here we isolated and characterized human APC10/Doc1, and found that APC10/Doc1 binds to APC core subunits throughout the cell cycle. Further, it was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in midbody in telophase and cytokinesis. These results strongly support the notion that human APC10/Doc1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

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Acknowledgements

We thank P Hieter for anti-Cdc27 and anti-Cdc16 antibodies, and Y Muro for anti-centoromere antibody. We also thank T Toda and T Oyamatsu for valuable discussion, and M Fujii and M Nishida for technical assistance. This work was supported in part by a Special Grant for Promotion of Research from the Institute of Physical and Chemical Research (RIKEN) and grants from the Ministry of Education, Science and Culture of Japan and from the Science Technology Agency of Japan.

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  1. Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1, Koyadai, Tsukuba, Ibaraki, 305-0074, Japan

    Yasuhiro Kurasawa & Kazuo Todokoro

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  1. Yasuhiro Kurasawa
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  2. Kazuo Todokoro
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Kurasawa, Y., Todokoro, K. Identification of human APC10/Doc1 as a subunit of anaphase promoting complex. Oncogene 18, 5131–5137 (1999). https://doi.org/10.1038/sj.onc.1203133

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