Differentiation between Eosinophils and Pseudo-eosinophils of Rabbits' Blood by means of a Peroxidase Reaction

Abstract

THE difficulty of distinguishing between eosinophil and pseudo-eosinophil leucocytes in rabbits' blood stained with the usual blood stains is well known. Ehrlich's triglycerin mixture, in which the eosinophil granules are shown up specifically in yellow, gives irregular results and, in general, rather unsatisfactory smears. Recently, I described a new technique for the peroxidase reaction on leucocytes in human blood films employing 2,6-dichlorphenol-indophenol¹. This technique applied to films of rabbits' blood allows to observer easily to single out the eosinophil leucocytes, even under relatively low power (for example, × 200).