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Nucleous and "Nucleolus Associated Chromatin" Acid Phosphatase Reaction

Abstract

The staining of nucleoli of liver and nervous tissue cells fixed in acetone and 80 per cent alcohol by Gomori's acid phosphatase technique has been reported1,2,3; a negative reaction was noted by another investigator4. In a study of the phosphatase reactions of smears of human bone marrow cells fixed in formol vapour5, it was reported that nucleoli did not stain after the acid phosphatase method, being surrounded by a chromatin ring strongly reminiscent of the "nucleolus associated chromatin" of Swedish workers6. This problem has been further investigated with animal material.

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RABINOVITCH, M. Nucleous and "Nucleolus Associated Chromatin" Acid Phosphatase Reaction. Nature 164, 878 (1949). https://doi.org/10.1038/164878a0

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