Electron-Microscopic Demonstration of a Membrane Structure Isolated from Nerve Tissue

Abstract

USING a technique of preparation similar to the one used for the analysis of the ultra-structure of the outer segment of retinal rods¹, it was possible to isolate a characteristic structure from the peripheral nerve tissue of the guinea pig. The sciatic nerve or motor roots of the spinal cord were fixed (24 hr.) in 2 per cent osmic acid, moderately fragmented by crushing between the ground-glass surfaces of a small glass joint by careful grinding and afterwards fragmented by sonic treatment using a frequency of 9 kc./s.