Abstract
RESEARCH on the excretion of urinary steroids has been handicapped by the lack of a procedure for hydrolysing steroidal conjugates without simultaneous partial destruction or transformation of the released steroids. We have therefore compared the urinary neutral 17-ketosteroids obtained by various methods of hydrolysis and extraction (for example, Talbot et al.1, Hamburger2, Beher and Gaebler3).
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References
Talbot, N. B., Butler, A. M., MacLachlan, E. A., and Jones, R. N., J. Biol. Chem., 136, 365 (1940).
Hamburger, C., Acta Endocrin. 1, 375 (1948).
Beher, W. T., and Gaebler, O. H., Anal. Chem., 23, 118 (1951).
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Talbot, N. B., Ryan, J., and Wolfe, J. K., J. Biol. Chem., 148, 593 (1943).
Bitman, J. and Cohen, S. L., J. Biol. Chem., 179, 455 (1949).
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JENSEN, C., TÖTTERMAN, L. An Improved Hydrolytic Procedure for Urinary Neutral 17-Ketosteroids. Nature 169, 374 (1952). https://doi.org/10.1038/169374a0
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DOI: https://doi.org/10.1038/169374a0
