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Successful large volume leukapheresis on a small infant allogeneic donor

Bone Marrow Transplantation volume 26, pages 339–341 (2000)Cite this article

Abstract

We successfully performed a hematopoietic stem cell apheresis on the smallest allogeneic donor reported to date, a 6.1 kg female. After placement of a dialysis catheter in the left femoral vein, the COBE Spectra was primed with one unit of paternal whole blood. Heparin and anticoagulant citrate dextrose, solution A (ACD-A) were slowly administered to the patient. Ionized calcium levels were checked hourly and calcium gluconate was given for hypocalcemia. Coagulation parameters were checked throughout the procedure. We collected 4.4 × 106 CD34+ cells/kg (recipient). The donor tolerated the procedure well and was discharged the following day. Five months later, the child manifests no obvious late effects. Bone Marrow Transplantation (2000) 26, 339–341.

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References

  1. Kanold J, Halle P, Berger M et al. Large-volume leukapheresis procedure for peripheral blood progenitor cell collection in children weighing 15kg or less: efficacy and safety evaluation Med Pediatr Oncol 1999 32: 7–10

    Article  CAS  Google Scholar 

  2. Berger M, Kanold J, Rapatel C et al. Feasibility of a PB CD34+ cell transplantation procedure using standard leukapheresis products in very small children Bone Marrow Transplant 1997 20: 191–198

    Article  CAS  Google Scholar 

  3. Takaue Y, Kawano Y, Abe T et al. Collection and transplantation of peripheral blood stem cells in very small children weighing 20kg or less Blood 1995 86: 372–380

    CAS  PubMed  Google Scholar 

  4. Nussbaumer W, Schonitzer D, Trieb T et al. Peripheral blood stem cell (PBSC) collection in extremely low-weight infants Bone Marrow Transplant 1996 18: 15–17

    CAS  PubMed  Google Scholar 

  5. Diaz M, Alegre A, Benito A et al. Peripheral blood progenitor cell collection by large-volume leukapheresis in low-weight children J Hematother 1998 7: 63–68

    Article  CAS  Google Scholar 

  6. Marson P, Petris M, De Silvestro G . Collection of peripheral blood stem cells in pediatric patients: a concise review on technical aspects Bone Marrow Transplant 1998 22: (Suppl. 5) S7–S11

    Article  Google Scholar 

  7. Gonzalez M, Benito A, Diaz M et al. Peripheral blood progenitor cell (PBPC) collection by large-volume leukapheresis from pediatric donors Bone Marrow Transplant 1999 23: 631–632

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Pediatric Blood and Marrow Transplant Program, Rainbow Babies and Children's Hospital and Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

    J Pahys, V Fisher & M Nieder

  2. Department of Transfusion Medicine, Apheresis Service, University Hospitals of Cleveland, Cleveland, Ohio, USA

    M Carneval, R Yomtovian & R Sarode

Authors
  1. J Pahys
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  2. V Fisher
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  3. M Carneval
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  4. R Yomtovian
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  5. R Sarode
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  6. M Nieder
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Pahys, J., Fisher, V., Carneval, M. et al. Successful large volume leukapheresis on a small infant allogeneic donor. Bone Marrow Transplant 26, 339–341 (2000). https://doi.org/10.1038/sj.bmt.1702482

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  • DOI: https://doi.org/10.1038/sj.bmt.1702482

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