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Progenitor Cell Mobilisation

Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells

Bone Marrow Transplantation volume 31, pages 17–22 (2003)Cite this article

Summary:

We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39–2.03%). After selection, the median purity and recovery was 94% (range 85.2–98.0%) and 69% (range 44–100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8–4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133−/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.

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Acknowledgements

This work was supported by the Assisi Foundation Memphis.

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Authors and Affiliations

  1. Division of Stem Cell Transplantation, St Jude Children's Research Hospital, Memphis, TN, USA

    P R Gordon, T Leimig, A Babarin-Dorner, J Houston, M Holladay, I Mueller, T Geiger & R Handgretinger

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  1. P R Gordon
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  2. T Leimig
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  3. A Babarin-Dorner
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Gordon, P., Leimig, T., Babarin-Dorner, A. et al. Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells. Bone Marrow Transplant 31, 17–22 (2003). https://doi.org/10.1038/sj.bmt.1703792

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