Abstract
IN earlier studies it has been shown1,2 that the very considerable concentration of choline in human semen (200–300 mgm./100 gm.) arises through the enzymatic hydrolysis by the prostate phosphatase of phosphoryl choline formed in the vesicular glands. In continuation of this investigation a method was worked out for the quantitative estimation of phosphoryl choline in tissue samples3. Choline is determined before and after treatment with phosphomonoesterase under optimal conditions. Purified prostate phosphatase is very useful for this purpose as it displays no activity towards secondary phosphate esters. On samples of tissue weighing 0.5 gm. it is possible to estimate amounts of phosphoryl choline corresponding to 10 mgm. choline chloride per 100 gm. of tissue with reasonable accuracy (± 5 per cent).
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LUNDQUIST, F. Glycerophosphoryl Choline as a Precursor of Free Choline in Mammalian Semen. Nature 172, 587–588 (1953). https://doi.org/10.1038/172587a0
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DOI: https://doi.org/10.1038/172587a0
