Abstract
MANNOSIDOSTREPTOMYCIN in some streptomycin preparations is conveniently detected by the paper chromatographic procedure of Winston and Eigen1. In the presence of high concentrations of salts such as in ion-exchange column eluates, or in fermentation broths, this procedure is not applicable: attempts to desalt such solutions, by mixed-bed ion-exchange, electrolytic de-ionization or solvent extraction, have proved unsuccessful. We have found it possible to separate streptomycin, mannosidostreptomycin and allied substances by paper electrophoresis, having developed (independently) a technique similar to that used for new antibiotic broths by Hosoya et al. 2, and King and Doery3.
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References
Winsten, W. A., and Eigen, E., J. Amer. Chem. Soc., 70, 3333 (1948).
Hosoya, S., Soeda, M., Komatsu, N., Hara, N., Sonoda, Y., and Arai, R., J. Antibiot. Jap., 4, 314 (1951).
King, N. K., and Doery, H. M., Nature, 171, 878 (1953).
Halliday, W. J., Nature, 169, 335 (1952).
Partridge, S. M., Biochem. J., 42, 238 (1948).
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FOSTER, M., ASHTON, G. Paper Electrophoresis of Streptomycins. Nature 172, 958–959 (1953). https://doi.org/10.1038/172958b0
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DOI: https://doi.org/10.1038/172958b0
