Abstract
IN connexion with recent electrophoretic studies of ox serum1, it became necessary to establish a reliable method for the fractionation of serum proteins which would allow numerous samples to be separated into distinct fractions of sufficient volume and concentration for subsequent physico-chemical and biological characterization. Zone electrophoresis2 appeared to be the method of choice, and the apparatus here described is a development of that of Svensson and Brattsten3, whose work should be consulted for the principles involved. The present modifications were introduced to overcome certain difficulties associated with their apparatus, notably the complexity of the feed arrangements and the lack of control of adverse pH and electrolytic changes.
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References
Bradish, C. J., Henderson, W. M., and Brooksby, J. B., Biochem. J., 56, 329, 335 (1954).
Tiselius, A., and Flodin, P., “Advances in Protein Chemistry”, 8, 461 (1953).
Svensson, H., and Brattsten, I., Arkiv Kemi, 1, 401 (1949).
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BRADISH, C., SMART, N. Fractionation of Serum Proteins by Zone Electrophoresis in Glass Powder. Nature 174, 272–273 (1954). https://doi.org/10.1038/174272a0
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DOI: https://doi.org/10.1038/174272a0
