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Role of Non-Protein Nitrogen in the Synthesis of Hæmoglobin in the Reticulocyte in vitro

Abstract

A CONSIDERABLE increase of non-protein nitrogen takes place when washed suspensions of red cells of anæmic rabbits, which are rich in reticulocytes, are incubated in physiological saline. This does not occur in suspensions from animals which are low in reticulocytes (see Table 1). The relation between increase of non-protein nitrogen and respiration, which is indicated by the data, is further borne out by the observations that: (a) increase of non-protein nitrogen is lower under anaerobic conditions (Table 2) ; (b) that it is inhibited by 2,4 dinitrophenol (Table 3).

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  1. Experimental results to be published.

  2. Coutelle, R., Rapoport, S., and Lindigkeit, R., Naturwiss., 42, 127 (1955).

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  3. Rapoport, S., Schweiger, H. G., and Strassner, W. L., Naturwiss., [43, 18 (1956)].

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SCHWEIGER, H., RAPOPORT, S. & SCHÖLZEL, E. Role of Non-Protein Nitrogen in the Synthesis of Hæmoglobin in the Reticulocyte in vitro . Nature 178, 141–142 (1956). https://doi.org/10.1038/178141b0

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