Abstract
Dedman, Farmer and Morris1 have shown that corticotropins can be oxidized by hydrogen peroxide to yield products which are inactive in the adrenal ascorbic acid depletion bioassay of Sayers, Sayers and Woodbury2, and that the biological activity can be completely restored by reduction with thiol compounds. The process is unaccompanied by any change in the amino-acid composition of the corticotropin (Dedman, Farmer and Morris, unpublished observations). Dixon and Stack-Dunne3 have confirmed this reaction, and have reported a separation of the oxidized and non-oxidized hormones by chromatography on the carboxylic acid cation exchange resin ‘Amberlite’ XE-64 (IRC-50).
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References
Dedman, M. L., Farmer, T. H., and Morris, C. J. O. R., Biochem. J., 59, XII (1955).
Sayers, M. A., Sayers, G., and Woodbury, L. A., Endocrin., 42, 379 (1948).
Dixon, H. B. F., and Stack-Dunne, M. P., Biochem. J., 61, 483 (1955).
Peterson, E. A., and Sober, H. A., J. Amer. Chem. Soc., 78, 751 (1956).
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FARMER, T., MORRIS, C. Separation of Oxidized from Non-Oxidized Corticotropin A1 by Chromatography on Carboxy-Methyl Cellulose. Nature 178, 1465–1466 (1956). https://doi.org/10.1038/1781465a0
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DOI: https://doi.org/10.1038/1781465a0
