Abstract
Weil-Malherbe and Bone1 claimed to have estimated the relative proportions of adrenaline and nor-adrenaline in mixtures of the two by the different colours of fluorescence given by these substances when coupled with ethylene diamine and extracted into iso-butanol. We attempted to repeat this work but obtained varying results. We therefore investigated further the mechanism of the reaction.
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References
Weil-Malherbe, H., and Bone, A. D., Biochem. J., 57, 311 (1952); Lancet, i, 974 (1953).
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BURN, G., FIELD, E. Fluorescence of Adrenaline and Nor-adrenaline with Ethylene Diamine. Nature 178, 542–543 (1956). https://doi.org/10.1038/178542a0
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DOI: https://doi.org/10.1038/178542a0
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