Candida albicans from Grass Leaves

Abstract

Candida albicans (Robin) Berkhout was recovered from a sample of grass leaves in the course of an examination of yeasts from the phyllosphere of pasture grasses. The sample, which was taken in December from an area that was lightly stocked with sheep, consisted of leaves of perennial ryegrass, white clover and flatweeds. Dilutions of the grass were made in 0.05 per cent (w/v) peptone water, plated on to acidified glucose peptone agar, and incubated at room temperature. Fifty yeasts were subcultured and identified, and three of these were the pathogen.