Abstract
LIVING hyphæ of Neurospora crassa were centrifuged and their contents stratified in distinct layers. Starting at the centrifugal end, the layers were as follows (Fig. 1): glycogen, ergastoplasm (microsomes), mitochondria, nuclei, ‘supernatant’ cytoplasm, vacuoles and fat. Each fraction could be identified by cytochemical reactions. It was found that most of the cytoplasmic ribonucleic acid resided in ergastoplasm, some in mitochondria, and none was detectable in the ‘supernatant’. Nuclei were relatively poor in ribonucleic acid.
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References
Ficq, A., Arch. Biol., 66, 509 (1955).
Brachet, J., “Chemical Cytology” (Academic Press, New York, 1957).
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ZALOKAR, M. Nuclear Origin of Ribonucleic Acid. Nature 183, 1330 (1959). https://doi.org/10.1038/1831330a0
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DOI: https://doi.org/10.1038/1831330a0
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