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Sub-fractionation of Human Serum Leucine Aminopeptidase

Nature volume 191, pages 1203–1204 (1961)Cite this article

Abstract

OUR earlier starch-gel electrophoretic zymograms1, obtained with 1-leucyl-β-naphthylamide (LBNA) substrate, revealed a single leucine aminopeptidase (LAP) zone for human serum. The substitution of alanyl-β-naphthylamide (ABNA) substrate now consistently reveals, within the diffuse trail behind the primary zone, an additional less-intense zone (Fig. 1a), detectable also for some sera by LBNA. Occasional sera give three or more zones; and one chronic pancreatitis serum has given a remarkable zymogram with five zones which, when observed through a suitable blue filter (Dupont No. 9 plastic ‘Varigam’ filter), can be distinguished as eight zones (Fig. 1b).

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References

  1. Dubbs, C. A., Vivonia, C., and Hilburn, J. M., Science, 131, 1529 (1960).

    Article  ADS  CAS  Google Scholar 

  2. Smithies, O., Biochem. J., 61, 629 (1955).

    Article  CAS  Google Scholar 

  3. Burstone, M. S., and Folk, J. E., J. Histochem. and Cytochem., 4, 217 (1956).

    Article  CAS  Google Scholar 

  4. Rutenburg, A. M., Goldbarg, J. A., and Pineda, E. P., New England J. Med., 259, 469 (1958).

    Article  CAS  Google Scholar 

  5. Arst, H. E., Manning, R. T., and Delp, M., Amer. J. Med. Sci., 238, 598 (1959).

    Article  CAS  Google Scholar 

  6. Pineda, E. P., Goldbarg, J. A., Banks, B. M., and Rutenburg, A. M., Gastroenterol., 38, 698 (1960).

    CAS  Google Scholar 

  7. Burstone, M. S., J. Nat. Cancer Inst., 16, 1149 (1956).

    PubMed  CAS  Google Scholar 

  8. Monis, B., Nachlas, M. M., and Seligman, A. M., Cancer, 12, 601 (1959).

    Article  CAS  Google Scholar 

  9. Wroblewski, F., Gregory, K., and Ross, C., Clin. Res., 8, 195 (1960).

    Google Scholar 

Download references

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Authors and Affiliations

  1. Veterans Administration Center and University of California Medical Center,

    CLYDE A. DUBBS

  2. University of California Medical Center, Los Angeles, California

    CHARLOTTE VIVONIA & JEAN M. HILBURN

Authors
  1. CLYDE A. DUBBS
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  2. CHARLOTTE VIVONIA
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  3. JEAN M. HILBURN
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DUBBS, C., VIVONIA, C. & HILBURN, J. Sub-fractionation of Human Serum Leucine Aminopeptidase. Nature 191, 1203–1204 (1961). https://doi.org/10.1038/1911203b0

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